ageratum yellow vein virus

Ageratum conyzoides L., a weed that occurs throughout southeast Asia, frequently exhibits yellow vein symptoms that are associated with infection by AYVV (7). Ageratum conyzoides L., a weed species widely distributed throughout southeast Asia, frequently exhibits striking yellow vein symptoms associated with infection by Ageratum yellow vein virus (AYVV), a member of the Geminiviridae (genus Begomovirus). We have shown that DNA β is biologically functional, being required for amplification of DNA A to high levels and for the efficient systemic spread of infection throughout A. conyzoides, and that it is essential for the induction of symptoms in this natural host. The remaining def17 nucleotides derive from DNA A (8). Ageratum Yellow vein China virus - OX1 Taxonomy ID: 1329380 (for references in articles please use NCBI:txid1329380) current name Several lines of evidence were presented confirming that AV3 promoter of AYVV-NT is active in E. coli and suggested that AV3 promoter might exhibit a level of activity in plant and yeast cells. The construction of clones pBinAYVdef17 and pBinAYVV1/7, containing partial repeats of a defective DNA A recombinant (referred to here as def17) and DNA1, respectively, in the binary vector pBin19, has been described (8, 10). PCR mediated amplification, cloning and sequencing of all begomovirus components from two plants from different fields showed the plants to be infected by Tomato yellow leaf curl virus (TYLCV) and Ageratum yellow vein virus (AYVV). This facilitated the isolation of a DNA 1 homologue from AYVV-infected A. conyzoides (10). Found inside – Page 152Alphasatellites replicate autonomously of their helper viruses in the nuclei of ... For example, the geminialphasatellite Ageratum yellow vein Singapore ... The positions of single-stranded (ss), supercoiled (sc), open-circular (oc), and dimeric single-stranded (ssd) DNAs are shown. Ageratum yellow vein virus in Guam. 2) failed to produce a systemic infection, demonstrating that DNA A alone is required for amplification of DNA β in plants. The preparation of viral supercoiled DNA (scDNA) from symptomatic A. conyzoides leaf material harvested in Singapore during July, 1995 (sample 1) has been described (8). In addition, two fragments containing begomovirus-alphasatellite sequences were cloned from sample AGLI4, and they were related to the defecting interfering molecule (Y14167) associated with Ageratum yellow vein virus from Asia. Both DNA A and DNA 1 encode a Rep and can autonomously replicate (5, 10). DNA β may have a direct effect on DNA A replication. REFERENCE STRAINS Ageratum yellow vein betasatellite Sequence | Genome | Proteome Host NATURAL HOSTS Dicotyledonous plants TROPISM Phloem and sieve or phloem-limited INTERACTIONS Cell receptors: - Host-virus interaction Ecology and disease GEOGRAPHY Asia, Africa, America ASSOCIATED DISEASES TRANSMISSION Vector=Insects VACCINE No ANTIVIRAL DRUGS A study explores how white bar formation in clownfish may be tied to differential recruitment in and adjustment to different sea anemone species. Pathogenicity of a natural recombinant associated with ageratum yellow vein disease: implications for geminivirus evolution and disease aetiology. 6, lanes 3 and 4). Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae, Occurrence and distribution of begomoviruses infecting tomatoes, peppers and cucurbits in Thailand, S Charoenvilaisiri, C Seepiban, N Phironrit… - Crop Protection, 2020 - Elsevier, Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum; Solanum subgen. 6). China Our data also demonstrate that the nanovirus-like DNA 1 component associated with infected A. conyzoides plays no essential role in the disease and represents a satellite-like DNA. X74516) from Singapore. Subsequently, the non-DNA A sequences of recombinant def19 were shown to be homologous to a region of a unique viral DNA, referred to as DNA 1, which had been isolated from cotton leaf curl virus-infected cotton (9). It can replicate autonomously, but relies on DNA A for coat protein (9) and for systemic infection of N. benthamiana (10). YC7-2 had the highest sequence identity (90.1%) with Ageratum yellow vein virus (AYVV-TW[TW:Tai:99], AF307861) and its betasatellite (96.5%) with Ageratum yellow vein betasatellite (AYVB-[TW:CHu:02], AJ542495 . The team at the John Innes Centre, led by Dr Keith Saunders and Professor George Lomonossoff, also developed a method for assembling geminivirus . Samples (5 μg of nucleic acids) were extracted from individual plants infected either by agroinoculation with DNA A and DNA β (lanes 1 and 2; sampled 26 days p.i.) Ageratum yellow vein virus. Furthermore, the satellite DNA previously found associated with tomato leaf curl virus is probably a defective DNA β homologue. PNAS is a partner of CHORUS, COPE, CrossRef, ORCID, and Research4Life. The C4 protein encoded by tomato leaf curl Yunnan virus reverses transcriptional gene silencing by interacting with NbDRM2 and impairing its DNA-binding ability. Abutting primers V4539 and V4540, corresponding to nucleotides 1,167–1,193 (virion-sense) and 1,139–1,166 (complementary-sense) of DNA β, were used to PCR-amplify full-length DNA β from scDNA sample 1. 1995 Dec;76 ( Pt 12):2915-22. The database accession numbers are indicated in each case. Subsequently, a similar role was established for cotton leaf curl virus (CLCuV) DNA β, which is part of a disease complex causing major crop losses in Pakistan. ⓘ Tobacco etch virus. Those symptoms suggested a virus-like disease. Found inside – Page 374Ageratum yellow vein Hualian virus - Hsinchu [Taiwan:Hsinchu:Tom:2003] Ageratum yellow vein Hualian virus - Hualian [Taiwan:Hualian 2:2000] Ageratum yellow ... curl Yunnan virus and . DNA β also contains the TAATATTAC motif and a stem-loop sequence between nucleotides 1,327–1,347/1–13. The data demonstrate that A. conyzoides plants exhibiting yellow vein disease contain a unique circular DNA and suggest that def17 has been produced by recombination between this DNA and AYVV DNA A. 6, lane 3). Found inside – Page 433However, in case of many other viruses, such as CLCuMuV and Kenaf leaf curl virus or ageratum yellow vein virus, the presence of betasatellite is necessary ... 1). Gnanasekaran P, Gupta N, Ponnusamy K, Chakraborty S. J Virol. 2004 Feb 4;98(2):121-9. doi: 10.1016/j.vetmic.2003.10.005. doi: 10.1371/journal.ppat.1008829. However, it is unable to induce typical yellow vein symptoms when reintroduced into A. conyzoides. PLoS Pathog. 6, lane 4) and by the inability to detect them by using the probe synthesized from the T3 promoter (Fig. ; References Badar U, Venkataraman S, AbouHaidar M, Hefferon K. Virus Genes. The positions of primers used to PCR-amplify DNA β fragments and the SpeI site used to clone DNA β are indicated. Elsewhere, A. conyzoides has been identified as host of Ageratum yellow vein virus. Ageratum yellow vein China virus: Ageratum yellow vein Taiwan virus: Soybean crinkle leaf virus: Propose photo. A sample from a symptomatic plant (1/10 of the sample in lane 1) has been included as a marker (lane M) to allow comparison of the relative levels of DNA A in symptomatic and asymptomatic plants. J Gen Virol. Samples were collected in 2011 from tomato plants exhibiting typical tomato leaf curl disease symptoms in the vicinity of Komae, Japan. Samples were fractionated by using agarose gels in TNE buffer, and blots were screened by using probes specific for DNA A (Top), DNA 1 (Middle), and DNA β (Bottom). A more intriguing possibility that cannot yet be ruled out is that DNA A and DNA β co-existed before the emergence of bipartite begomoviruses, and that DNA B usurped DNA β by a similar mechanism. The complete nucleotide sequence of the DNA-A shared 99.9% identity with that of the AYVV . AbstractAgeratum yellow vein disease (AYVD) is caused by the geminivirus ageratum yellow vein virus (AYVV) and an associated DNA β satellite. Found inside – Page 673... as the presence of ageratum yellow vein virus betasatellite facilitates the bipartite begomovirus Sri Lankan cassava mosaic virus to infect and produce ... Ageratum yellow vein virus. The genome of the virus was 2737 nucleotides in length (KP188831) and exhibited an organization like that of other monopartite begomoviruses, sharing the highest nucleotide sequence similarity (87.7% identity) with ageratum yellow vein virus (AM940137). Mutagenesis studies and transcript mapping are in progress to investigate gene expression from this component. Ageratum yellow vein China virus-associated DNA beta complete genome, isolate G86. The virus, ageratum yellow vein virus (AYVV), has been identified as a geminivirus on the basis of host range, transmission, virion morphology, serology and cytopathology (Tan & Wong, 1993; Wong . So far, no other unique nanovirus-like DNA component has been isolated from infected A. conyzoides. Abstract Samples were collected in 2011 from tomato plants exhibiting typical tomato leaf curl disease symptoms in the vicinity of Komae, Japan. The amplified DNA was cloned into pGEM-T Easy (Promega), and six clones were chosen at random and sequenced. DNA A and DNA B of bipartite begomoviruses share a common region of ≈200 nucleotides, primarily located within the intergenic region, that contains cis-acting elements for replication and the control of gene expression. Found inside(1997) have also shown the presence of a naturally-occurring DI-DNA in Ageratum yellow vein virus (geminivirus) infections of Ageratum conyzoides. Regions of the recombinant def17 derive from DNA A (black) and DNA β (gray) sequences, the coordinates of which are indicated. Found inside – Page 210ssDNA satellites (circular) Ageratum yellow vein virus (AYVV)/AYVV satellite DNA fl 1347 118/13.7 AJ25.2072 Bhendi yellow vein mosaic virus (BYVMV)/BYVMV ... Insights into the functional characteristics of geminivirus rolling-circle replication initiator protein and its interaction with host factors affecting viral DNA replication. Most begomoviruses have bipartite genomes (DNAs A and B), but only a DNA A has been identified for AYVV. DNA beta depends on DNA A for replication and is encapsidated by DNA A-encoded coat protein and so has characteristics of a DNA satellite. The team studied a type of geminvirus called ageratum yellow vein virus, which was produced in tobacco plants under carefully controlled conditions by researchers at the John Innes Centre in Norwich. 2021 Feb;57(1):1-22. doi: 10.1007/s11262-020-01806-9. Clipboard, Search History, and several other advanced features are temporarily unavailable. Tomato farms in the Dodoma region of Tanzania where a high incidence of Tomato yellow leaf curl Tanzania virus has been reported were visited to survey for weed hosts. Samples (5 μg of…, Infectivity of DNA A and DNA β in A. conyzoides . Ageratum conyzoides L., a weed species widely distributed throughout southeast Asia, frequently exhibits striking yellow vein symptoms associated with infection by Ageratum yellow vein virus (AYVV), a member of the Geminiviridae (genus Begomovirus).Most begomoviruses have bipartite genomes (DNAs A and B), but only a DNA A has been identified for AYVV In two separate experiments, individual infected plants were used to transmit the disease to healthy A. conyzoides seedlings using B. tabaci. The position of single-stranded DNA (ss) is shown. 1995 Dec;76 ( Pt 12):2915-22. Thus, DNA β may have become associated with DNA A during co-infection by a begomovirus and a nanovirus, making DNA B (if present) functionally redundant. Field evaluation of commercial tomato cultivars against . DNA A encodes a replication-associated protein (Rep), coat protein, and proteins that participate in the control of replication and gene expression. Blots were screened by using strand-specific probes synthesized in vitro from pBS-AYVVβ using the T7 or T3 promoter as indicated. First report of Malvastrum yellow vein virus infecting Ageratum conyzoides T. Jiang Institute of Biotechnology, Zhejiang University, Hangzhou 310029, P.R. It has been established that AYVV DNA A is associated with infected A. conyzoides plants, but attempts to isolate a component equivalent to DNA B have been unsuccessful (5, 8). DNA was transferred to Hybond-N (Amersham Pharmacia) and was hybridized to randomly labeled probes (18) specific for DNA A, DNA β, and DNA 1. In 2007 a unique strain was found on Guam.. See also. During a survey in February 2011, severe symptoms of upward leaf curling, vein enation on lower side of the leaves, and shortening of internodes were observed on 20 out of 117 Amaranthus hypochondriacus plants (17% disease incidence) examined at breeding plots of CSIR-NBRI, Lucknow. PCR with begomovirus-specific primers (3) produced the . The construction of clones pHNIC419 and pHNBin419 containing partial repeats of AYVV DNA A in pIC19H and the binary vector pBin19, respectively, has been described (5). TLCV DNA A alone can infect tomato, N. tabacum, and Datura stramonium (4), indicating that a functional DNA β is not essential, at least in these solanaceous species. A region of DNA β between virion-sense nucleotides 704–972 is particularly A-rich (64%). (B) The program frames (13) was used to predict DNA β ORFs, starting from an in-frame ATG triplet, that occur in the virion-sense (top) and complementary-sense (bottom) strands. 1, was deduced from the ability to detect ssDNA using only the probe synthesized from the T7 promoter together with a knowledge of the orientation of the DNA β insert in pBS-AYVVβ. Found insideGeminiviridae Begomovirus: (African cassava mosaic virus–ACMV/ssDNA): Abutilon mosaic virus (AbMV); Ageratum enation virus (AEV); Ageratum yellow vein china ... Molecular interactions of plant viral satellites. Rigorous attempts to isolate a DNA B component have been unsuccessful, and it was concluded that another factor must contribute to the disease (5). Samples were extracted 3, 5, and 7 days p.i. Encapsidation is required for whitefly transmission and may be necessary for systemic movement of the viral DNA, as has been suggested for other monopartite begomoviruses (25, 26). Most begomoviruses have bipartite genomes (DNAs A and B), but only a DNA A has been identified for AYVV. Mansoor S, Khan SH, Hussain M, Zafar Y, Pinner MS, Briddon RW, Stanley J, Markham PG. The most abundant transcript 5′-terminus is located 8 bases upstream of the βC1 ORF putative initiation codon . Found inside – Page 31... gene silencing encoded by Ageratum yellow vein virus, a monopartite begomovirus. Virus Res 149(1):19–27 Shih SL, Tsai WS, Green SK, Khalid S, Ahmad I, ... 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